The development and validation of a fast and robust dried blood spot based lipid profiling method to study infant metabolism.

ABSTRACT: Early life exposures and metabolic programming are associated with later disease risk. In particular lipid metabolism is thought to play a key role in the development of the metabolic syndrome and insulin resistance in later life. Investigative studies of metabolic programming are limited by the ethics and practicalities of sample collection in small infants. Dried blood spots on filter paper, derived from heel pricks are considered as the most suitable option for this age group. We validated a novel lipid profiling method, based on high resolution mass spectrometry to successfully determine the lipid composition of infants using dried blood spots. The spotting and air drying of blood on paper has noticeable effects on many of the lipids, leading to lipid oxidation and hydrolysis, which de

SUBMITTER: Koulman A 

PROVIDER: S-EPMC4145199 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature