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Gene silencing via DNA methylation in naturally occurring Tragopogon miscellus (Asteraceae) allopolyploids.


ABSTRACT: BACKGROUND: Hybridization coupled with whole-genome duplication (allopolyploidy) leads to a variety of genetic and epigenetic modifications in the resultant merged genomes. In particular, gene loss and gene silencing are commonly observed post-polyploidization. Here, we investigated DNA methylation as a potential mechanism for gene silencing in Tragopogon miscellus (Asteraceae), a recent and recurrently formed allopolyploid. This species, which also exhibits extensive gene loss, was formed from the diploids T. dubius and T. pratensis. RESULTS: Comparative bisulfite sequencing revealed CG methylation of parental homeologs for three loci (S2, S18 and TDF-44) that were previously identified as silenced in T. miscellus individuals relative to the diploid progenitors. One other locus (S3) examined did not show methylation, indicating that other transcriptional and post-transcriptional mechanisms are likely responsible for silencing that homeologous locus. CONCLUSIONS: These results indicate that Tragopogon miscellus allopolyploids employ diverse mechanisms, including DNA methylation, to respond to the potential shock of genome merger and doubling.

SUBMITTER: Sehrish T 

PROVIDER: S-EPMC4148530 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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Gene silencing via DNA methylation in naturally occurring Tragopogon miscellus (Asteraceae) allopolyploids.

Sehrish Tina T   Symonds V Vaughan VV   Soltis Douglas E DE   Soltis Pamela S PS   Tate Jennifer A JA  

BMC genomics 20140822


<h4>Background</h4>Hybridization coupled with whole-genome duplication (allopolyploidy) leads to a variety of genetic and epigenetic modifications in the resultant merged genomes. In particular, gene loss and gene silencing are commonly observed post-polyploidization. Here, we investigated DNA methylation as a potential mechanism for gene silencing in Tragopogon miscellus (Asteraceae), a recent and recurrently formed allopolyploid. This species, which also exhibits extensive gene loss, was forme  ...[more]

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