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Synthesis of non-linear protein dimers through a genetically encoded Thiol-ene reaction.

ABSTRACT: Site-specific incorporation of bioorthogonal unnatural amino acids into proteins provides a useful tool for the installation of specific functionalities that will allow for the labeling of proteins with virtually any probe. We demonstrate the genetic encoding of a set of alkene lysines using the orthogonal PylRS/PylTCUA pair in Escherichia coli. The installed double bond functionality was then applied in a photoinitiated thiol-ene reaction of the protein with a fluorescent thiol-bearing probe, as well as a cysteine residue of a second protein, showing the applicability of this approach in the formation of heterogeneous non-linear fused proteins.

SUBMITTER: Torres-Kolbus J

PROVIDER: S-EPMC4152134 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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Synthesis of non-linear protein dimers through a genetically encoded Thiol-ene reaction.

Torres-Kolbus Jessica J Chou Chungjung C Liu Jihe J Deiters Alexander A

PloS one 20140902 9

Site-specific incorporation of bioorthogonal unnatural amino acids into proteins provides a useful tool for the installation of specific functionalities that will allow for the labeling of proteins with virtually any probe. We demonstrate the genetic encoding of a set of alkene lysines using the orthogonal PylRS/PylTCUA pair in Escherichia coli. The installed double bond functionality was then applied in a photoinitiated thiol-ene reaction of the protein with a fluorescent thiol-bearing probe, a ...[more]

PMID: 25181502

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