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Maternally acquired genotoxic Escherichia coli alters offspring's intestinal homeostasis.

ABSTRACT: The neonatal gut is rapidly colonized by a newly dominant group of commensal Escherichia coli strains among which a large proportion produces a genotoxin called colibactin. In order to analyze the short- and long-term effects resulting from such evolution, we developed a rat model mimicking the natural transmission of E. coli from mothers to neonates. Genotoxic and non-genotoxic E. coli strains were equally transmitted to the offspring and stably colonized the gut across generations. DNA damage was only detected in neonates colonized with genotoxic E. coli strains. Signs of genotoxic stress such as anaphase bridges, higher occurrence of crypt fission and accelerated renewal of the mature epithelium were detected at adulthood. In addition, we observed alterations of secretory cell populations and gut epithelial barrier. Our findings illustrate how critical is the genotype of E. coli strains acquired at birth for gut homeostasis at adulthood.

SUBMITTER: Payros D

PROVIDER: S-EPMC4153768 | biostudies-literature | 2014 May-Jun

REPOSITORIES: biostudies-literature

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Maternally acquired genotoxic Escherichia coli alters offspring's intestinal homeostasis.

Payros Delphine D Secher Thomas T Boury Michèle M Brehin Camille C Ménard Sandrine S Salvador-Cartier Christel C Cuevas-Ramos Gabriel G Watrin Claude C Marcq Ingrid I Nougayrède Jean-Philippe JP Dubois Damien D Bedu Antoine A Garnier Fabien F Clermont Olivier O Denamur Erick E Plaisancié Pascale P Theodorou Vassilia V Fioramonti Jean J Olier Maïwenn M Oswald Eric E

Gut microbes 20140501 3

The neonatal gut is rapidly colonized by a newly dominant group of commensal Escherichia coli strains among which a large proportion produces a genotoxin called colibactin. In order to analyze the short- and long-term effects resulting from such evolution, we developed a rat model mimicking the natural transmission of E. coli from mothers to neonates. Genotoxic and non-genotoxic E. coli strains were equally transmitted to the offspring and stably colonized the gut across generations. DNA damage ...[more]

PMID: 24971581

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Dataset Information

Maternally acquired genotoxic Escherichia coli alters offspring's intestinal homeostasis.

Publications

Maternally acquired genotoxic Escherichia coli alters offspring's intestinal homeostasis.

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