Ontology highlight
ABSTRACT:
SUBMITTER: Kummer L
PROVIDER: S-EPMC4154710 | biostudies-literature | 2013 Jun
REPOSITORIES: biostudies-literature
Kummer Lutz L Hsu Chia-Wen CW Dagliyan Onur O MacNevin Christopher C Kaufholz Melanie M Zimmermann Bastian B Dokholyan Nikolay V NV Hahn Klaus M KM Plückthun Andreas A
Chemistry & biology 20130601 6
Investigation of protein activation in living cells is fundamental to understanding how proteins are influenced by the full complement of upstream regulators they experience. Here, we describe the generation of a biosensor based on the DARPin binding scaffold suited for intracellular applications. Combining library selection and knowledge-based design, we created an ERK activity biosensor by derivatizing a DARPin specific for phosphorylated ERK with a solvatochromatic merocyanine dye, whose fluo ...[more]