Mechanism of action at the molecular level of the antiviral drug 3(2H)-isoflavene against type 2 poliovirus.
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ABSTRACT:
SUBMITTER: Salvati AL
PROVIDER: S-EPMC415587 | biostudies-literature | 2004 Jun
REPOSITORIES: biostudies-literature
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Mechanism of action at the molecular level of the antiviral drug 3(2H)-isoflavene against type 2 poliovirus.
Salvati Anna L AL De Dominicis Alessandra A Tait Sabrina S Canitano Andrea A Lahm Armin A Fiore Lucia L
Antimicrobial agents and chemotherapy 20040601 6
The mechanism of action of the antiviral compound 3(2H)-isoflavene against Sabin type 2 poliovirus has been studied, and interference with virus uncoating was demonstrated. Isolation and sequencing of drug-resistant variants revealed single amino acid substitutions (I194M or D131V) in the VP1 capsid protein. While M194 is located in a hydrophobic pocket and should partially fill the space occupied by the isoflavene ring, V131 is exposed on the VP1 surface, forming a contact with VP4. The D131V m ...[more]
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