Ontology highlight
ABSTRACT:
SUBMITTER: Stetter FWS
PROVIDER: S-EPMC4156670 | biostudies-literature | 2014 Sep
REPOSITORIES: biostudies-literature
Stetter Frank W S FWS Cwiklik Lukasz L Jungwirth Pavel P Hugel Thorsten T
Biophysical journal 20140901 5
Cholesterol is important for the formation of microdomains in supported lipid bilayers and is enriched in the liquid-ordered phase. To understand the interactions leading to this enrichment, we developed an AFM-based single-lipid-extraction (SLX) approach that enables us to determine the anchoring strength of cholesterol in the two phases of a phase-separated lipid membrane. As expected, the forces necessary for extracting a single cholesterol molecule from liquid-ordered phases are significantl ...[more]