Ontology highlight
ABSTRACT:
SUBMITTER: Macmillan H
PROVIDER: S-EPMC4157100 | biostudies-literature | 2014 Sep
REPOSITORIES: biostudies-literature
Macmillan Henriette H Strohman Michael J MJ Ayyangar Sashi S Jiang Wei W Rajasekaran Narendiran N Spura Armin A Hessell Ann J AJ Madec Anne-Marie AM Mellins Elizabeth D ED
Journal of immunology (Baltimore, Md. : 1950) 20140806 6
B cells internalize extracellular Ag into endosomes using the Ig component of the BCR. In endosomes, Ag-derived peptides are loaded onto MHC class II proteins. How these pathways intersect remains unclear. We find that HLA-DM (DM), a catalyst for MHC class II peptide loading, coprecipitates with Ig in lysates from human tonsillar B cells and B cell lines. The molecules in the Ig/DM complexes have mature glycans, and the complexes colocalize with endosomal markers in intact cells. A larger fracti ...[more]