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Reverse genetic analysis of the transcription regulatory sequence of the coronavirus transmissible gastroenteritis virus.

ABSTRACT: Coronavirus discontinuous transcription uses a highly conserved sequence (CS) in the joining of leader and body RNAs. Using a full-length infectious construct of transmissable gastroenteritis virus, the present study demonstrates that subgenomic transcription is heavily influenced by upstream flanking sequences and supports a mechanism of transcription attenuation that is regulated in part by a larger domain composed of primarily upstream flanking sequences which select appropriately positioned CS elements for synthesis of subgenomic RNAs.

SUBMITTER: Curtis KM

PROVIDER: S-EPMC415797 | biostudies-literature | 2004 Jun

REPOSITORIES: biostudies-literature

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Reverse genetic analysis of the transcription regulatory sequence of the coronavirus transmissible gastroenteritis virus.

Curtis Kristopher M KM Yount Boyd B Sims Amy C AC Baric Ralph S RS

Journal of virology 20040601 11

Coronavirus discontinuous transcription uses a highly conserved sequence (CS) in the joining of leader and body RNAs. Using a full-length infectious construct of transmissable gastroenteritis virus, the present study demonstrates that subgenomic transcription is heavily influenced by upstream flanking sequences and supports a mechanism of transcription attenuation that is regulated in part by a larger domain composed of primarily upstream flanking sequences which select appropriately positioned ...[more]

PMID: 15141005

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