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Isolation and characterization of Babesia pecorum sp. nov. from farmed red deer (Cervus elaphus).

ABSTRACT: The diversity of Babesia species infecting cervids in parts of central and southern Spain was analyzed by collecting blood from farmed red deer (Cervus elaphus). Babesia sp. was isolated in vitro from two red deer herds in Cádiz and Ciudad Real. The number of Babesia sp. carriers differed between the two herds: 36/77 in Cádiz and 1/35 in Ciudad Real. Hyalomma lusitanicum was the most prevalent tick species identified on the Cádiz farm vegetation and on sampled animals, and is therefore a candidate vector. The molecular characteristics of 21 isolates were determined by complete (8 isolates) or partial (13 isolates) 18S rRNA gene sequencing. The sequences were highly similar (over 99.4% identity) and 6 sequence types were identified at the level of one herd only, demonstrating a rather high genetic diversity. They formed a monophyletic clade, and members of the three main sequence types shared a similar morphology and the same erythrocyte susceptibility pattern. This clade also included Babesia sp. Xinjiang isolated from sheep in China and Babesia sp. identified in giraffe in South Africa, with identities higher than 98.3% and statistically relevant phylogenetic support. None of the biological properties analyzed for both Babesia from red deer and Babesia sp. Xinjiang allowed their differentiation (ability to develop in vitro in erythrocytes from cattle and sheep, as well as in erythrocytes from different cervids, unsuccessful infection of calves). We propose the Babesia isolated from red deer as a new species named B. pecorum. Whether Babesia sp. Xinjiang and the Babesia characterized in South Africa belong to the same species is debated.

SUBMITTER: Jouglin M

PROVIDER: S-EPMC4158131 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature

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Anaplasma phagocytophilum and Babesia Species of Sympatric Roe Deer (Capreolus capreolus), Fallow Deer (Dama dama), Sika Deer (Cervus nippon) and Red Deer (Cervus elaphus) in Germany.

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| S-EPMC7699845 | biostudies-literature

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Body composition and venison quality of farmed red deer (Cervus elaphus) hinds reared on grass, papilionaceous or mixed pasture paddocks.

Project description:Red deer (Cervus elaphus) hinds ( n=3×10 ) of identical initial body weight (BW, ca. 68?kg) were reared on a monocotyledonous grass (G group), on a grass-papilionaceous (GP group) or on pure papilionaceous pasture each of 2?ha (P group) for 219 d. At the end of the experiment carcass tissue composition was assessed by means of computer tomography, slaughter value and meat quality were characterized and tissue - longissimus thoracis et lumborum (LTL), thigh and liver - samples were taken for fatty acid composition analysis. The primary aim was to assess nutrition-driven differences. Hinds of group P provided higher final BW (101?kg vs. 90 and 91.9?kg in groups G and GP, respectively) and higher BW gain (32.6?kg during the total period vs. 22.4 and 22.1?kg). The carcass weight exceeded those of the other groups significantly (68.8?kg vs. 59.3 and 63.2?kg), while there was no difference among groups in the perirenal fat weight and red color tone ( a* ) of the LTL. Groups G and P differed significantly in the LTL weight (highest in P), its dripping loss (lowest in G), lightness (L; highest in P) and yellow color tone ( b* ). In the thigh muscle, LTL and liver the highest proportion of fatty acid CLA9c11t was reached on the G pasture, and the same trend was true for docosahexaenoic acid (DHA , C22:6 n3) in the muscles. The n6? / ?n3 fatty acid ratio was the highest on the P pasture in the liver and both muscles. The liver incorporated the highest proportion of linoleic acid (C18:2 n6) and converted it rather effectively to arachidonic acid (C20:4 n6), coupled with the lowest ? -linolenic acid presence. In conclusion, concerning muscle mass production, group P proved to be the most advantageous pasture; meanwhile LTL meat quality factors (dripping loss, DHA proportion, pH, color) were more favorable on the G pasture.

| S-EPMC6852854 | biostudies-literature

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