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Action potential initiation in neocortical inhibitory interneurons.


ABSTRACT: Action potential (AP) generation in inhibitory interneurons is critical for cortical excitation-inhibition balance and information processing. However, it remains unclear what determines AP initiation in different interneurons. We focused on two predominant interneuron types in neocortex: parvalbumin (PV)- and somatostatin (SST)-expressing neurons. Patch-clamp recording from mouse prefrontal cortical slices showed that axonal but not somatic Na+ channels exhibit different voltage-dependent properties. The minimal activation voltage of axonal channels in SST was substantially higher (?7 mV) than in PV cells, consistent with differences in AP thresholds. A more mixed distribution of high- and low-threshold channel subtypes at the axon initial segment (AIS) of SST cells may lead to these differences. Surprisingly, NaV1.2 was found accumulated at AIS of SST but not PV cells; reducing NaV1.2-mediated currents in interneurons promoted recurrent network activity. Together, our results reveal the molecular identity of axonal Na+ channels in interneurons and their contribution to AP generation and regulation of network activity.

SUBMITTER: Li T 

PROVIDER: S-EPMC4159120 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature

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Action potential initiation in neocortical inhibitory interneurons.

Li Tun T   Tian Cuiping C   Scalmani Paolo P   Frassoni Carolina C   Mantegazza Massimo M   Wang Yonghong Y   Yang Mingpo M   Wu Si S   Shu Yousheng Y  

PLoS biology 20140909 9


Action potential (AP) generation in inhibitory interneurons is critical for cortical excitation-inhibition balance and information processing. However, it remains unclear what determines AP initiation in different interneurons. We focused on two predominant interneuron types in neocortex: parvalbumin (PV)- and somatostatin (SST)-expressing neurons. Patch-clamp recording from mouse prefrontal cortical slices showed that axonal but not somatic Na+ channels exhibit different voltage-dependent prope  ...[more]

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