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Characterization of myxobacterial A-motility: insights from microcinematographic observations.

ABSTRACT: Myxococcus xanthus, a predatory soil bacterium, has long been used as a model organism to study bacterial gliding motility. Research has revealed that two fundamentally distinct motor systems power gliding in this bacterium: repeated extensions and retractions of pili mediate social or (S-) motility, whereas the motor powering adventurous or (A-) motility has not yet been identified with certainty. Several different hypotheses to explain A-motility have been suggested and differ with respect to the involved motor structures as well as the mechanics of motility. As some of the more recent models invoke helically arranged structures and processes that require rotations of the cell, we decided to re-examine myxobacterial motility using microcinematographic techniques. This re-examination was also prompted by the lack of direct experimental data on the rotation of M. xanthus during gliding. Microcinematographic observations of deformed cells and cells containing large stationary intracellular structures reveal clearly that M. xanthus gliding does not require cell rotation.

SUBMITTER: Koch MK

PROVIDER: S-EPMC4160302 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature

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Characterization of myxobacterial A-motility: insights from microcinematographic observations.

Koch Matthias K MK Hoiczyk Egbert E

Journal of basic microbiology 20130115 9

Myxococcus xanthus, a predatory soil bacterium, has long been used as a model organism to study bacterial gliding motility. Research has revealed that two fundamentally distinct motor systems power gliding in this bacterium: repeated extensions and retractions of pili mediate social or (S-) motility, whereas the motor powering adventurous or (A-) motility has not yet been identified with certainty. Several different hypotheses to explain A-motility have been suggested and differ with respect to ...[more]

PMID: 23322594

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Project description:Chemosensory systems (CSS) are among the most complex organizations of proteins functioning cooperatively to regulate bacterial motility and other cellular activities. These systems have been studied extensively in bacteria, and usually, they are present as a single system. Eight CSS, the highest number in bacteria, have been reported in Myxococcus xanthus DK1622 and are involved in coordinating diverse functions. Here, we have explored and compared the CSS in all available genomes of order Myxococcales. Myxococcales members contain 97 to 476 two-component system (TCS) proteins, which assist the bacteria in surviving and adapting to varying environmental conditions. The number of myxobacterial CSS ranges between 1 and 12, with the largest number in family Cystobacteraceae and the smallest in Nannocystaceae CheA protein was used as a phylogenetic marker to infer evolutionary relatedness between different CSS, and six novel CSS ("extra CSS" [ECSS]) were thus identified in the myxobacteria besides the previously reported Che1 to Che8 systems from M. xanthus Che1 to Che8 systems are monophyletic to deltaproteobacteria, whereas the newly identified ECSS form separate clades with different bacterial classes. The comparative modular organization was concordant with the phylogeny. Four clusters lacking CheA proteins were also identified via CheB-based phylogenetic analysis and were categorized as accessory CSS (ACSS). In Archangium, an orphan CSS was identified, in which both CheA and CheB were absent. The novel, accessory, and orphan multimodular CSS identified here suggest the emergence of myxobacterial CSS and could assist in further characterizing their roles.IMPORTANCE This study is focused on chemosensory systems (CSS), which help the bacterium in directing its movement toward or away from chemical gradients. CSS are present as a single system in most of the bacteria except in some groups, including Myxococcus xanthus, which has 8 CSS, the highest number reported to date. This is the first comprehensive study carrying out a comparative analysis of the 22 available myxobacterial genomes, which suggests the evolutionary diversity of these systems. We are interested in understanding the distribution of CSS within all known myxobacteria and their probable evolution.

Dataset Information

Characterization of myxobacterial A-motility: insights from microcinematographic observations.

Publications

Characterization of myxobacterial A-motility: insights from microcinematographic observations.

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