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High-throughput bead-based identification of structure-switching aptamer beacons.

ABSTRACT: We describe a new platform to identify structure-switching DNA beacon aptamers, which detect small molecules in a specific manner. By clonally amplifying a DNA library designed to fluoresce in response to binding events onto microbeads, aptamer beacons can be selected by stringent fluorescence-assisted sorting. We validated this method by isolating known and novel anti-steroid aptamers from two separate DNA libraries that were structurally enriched with three-way junctions. Importantly, aptamers were retrieved in only a few (three) rounds of selection by this approach and did not require further optimization, significantly streamlining the process of beacon development.

SUBMITTER: Trevino SG

PROVIDER: S-EPMC4161366 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature

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High-throughput bead-based identification of structure-switching aptamer beacons.

Trevino Simon G SG Levy Matthew M

Chembiochem : a European journal of chemical biology 20140723 13

We describe a new platform to identify structure-switching DNA beacon aptamers, which detect small molecules in a specific manner. By clonally amplifying a DNA library designed to fluoresce in response to binding events onto microbeads, aptamer beacons can be selected by stringent fluorescence-assisted sorting. We validated this method by isolating known and novel anti-steroid aptamers from two separate DNA libraries that were structurally enriched with three-way junctions. Importantly, aptamers ...[more]

PMID: 25056925

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