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Assignments of RNase A by ADAPT-NMR and enhancer.


ABSTRACT: We report here backbone (1)H and (15)N assignments for ribonuclease A obtained by using ADAPT-NMR, a fully-automated approach for combined data collection, spectral analysis and resonance assignment. ADAPT-NMR was able to assign 98% of the resonances with 93% agreement with traditional data collection and assignment. Further refinement of the automated results with ADAPT-NMR enhancer led to complete (100%) assignments with 96% agreement with assignments by the traditional approach.

SUBMITTER: Tonelli M 

PROVIDER: S-EPMC4162851 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature

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Assignments of RNase A by ADAPT-NMR and enhancer.

Tonelli Marco M   Eller Chelcie H CH   Singarapu Kiran K KK   Lee Woonghee W   Bahrami Arash A   Westler William M WM   Raines Ronald T RT   Markley John L JL  

Biomolecular NMR assignments 20140312 1


We report here backbone (1)H and (15)N assignments for ribonuclease A obtained by using ADAPT-NMR, a fully-automated approach for combined data collection, spectral analysis and resonance assignment. ADAPT-NMR was able to assign 98% of the resonances with 93% agreement with traditional data collection and assignment. Further refinement of the automated results with ADAPT-NMR enhancer led to complete (100%) assignments with 96% agreement with assignments by the traditional approach. ...[more]

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