Dataset Information

Real time ligand-induced motion mappings of AChBP and nAChR using X-ray single molecule tracking.

ABSTRACT: We observed the dynamic three-dimensional (3D) single molecule behaviour of acetylcholine-binding protein (AChBP) and nicotinic acetylcholine receptor (nAChR) using a single molecule tracking technique, diffracted X-ray tracking (DXT) with atomic scale and 100??s time resolution. We found that the combined tilting and twisting motions of the proteins were enhanced upon acetylcholine (ACh) binding. We present the internal motion maps of AChBP and nAChR in the presence of either ACh or ?-bungarotoxin (?Btx), with views from two rotational axes. Our findings indicate that specific motion patterns represented as biaxial angular motion maps are associated with channel function in real time and on an atomic scale.

SUBMITTER: Sekiguchi H

PROVIDER: S-EPMC4165275 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature

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Real time ligand-induced motion mappings of AChBP and nAChR using X-ray single molecule tracking.

Sekiguchi Hiroshi H Suzuki Yasuhito Y Nishino Yuri Y Kobayashi Suzuko S Shimoyama Yoshiko Y Cai Weiyan W Nagata Kenji K Okada Masato M Ichiyanagi Kouhei K Ohta Noboru N Yagi Naoto N Miyazawa Atsuo A Kubo Tai T Sasaki Yuji C YC

Scientific reports 20140916

We observed the dynamic three-dimensional (3D) single molecule behaviour of acetylcholine-binding protein (AChBP) and nicotinic acetylcholine receptor (nAChR) using a single molecule tracking technique, diffracted X-ray tracking (DXT) with atomic scale and 100 μs time resolution. We found that the combined tilting and twisting motions of the proteins were enhanced upon acetylcholine (ACh) binding. We present the internal motion maps of AChBP and nAChR in the presence of either ACh or α-bungaroto ...[more]

PMID: 25223459

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Project description:PurposeThe objective of this work is to test the validity of the Gaussian approximation for prostate motion through characterization of its spatial distribution.Methods and materialsReal-time intrafraction prostate motion was observed using Calypso 4-dimensional (4D) nonradioactive electromagnetic tracking system. We report the results from a total of 1024 fractions from 31 prostate cancer patients. First, the correlation of prostate motion in right/left (RL), anteroposterior (AP), and superoinferior (SI) direction were determined using Pearson's correlation of coefficient. Then the spatial distribution of prostate motion was analyzed for individual fraction, individual patient including all fractions, and all patients including all fractions. The displacement in RL, AP, SI, oblique, or total direction is fitted into a Gaussian distribution, and a Lilliefors test was used to evaluate the validity of the hypothesis that the displacement is normally distributed.ResultsThere is high correlation in AP/SI direction (61% of fractions with medium or strong correlation). This is consistent with the longitudinal oblique motion of the prostate, and likely the effect from respiration on an organ confined within the genitourinary diaphragm with the rectum sitting posteriorly and bladder sitting superiorly. In all directions, the non-Gaussian distribution is more common for individual fraction, individual patient including all fractions, and all patients including all fractions. The spatial distribution of prostate motion shows an elongated shape in oblique direction, indicating a higher range of motion in the AP and SI directions.ConclusionsOur results showed that the prostate motion is highly correlated in AP and SI direction, indicating an oblique motion preference. In addition, the spatial distribution of prostate motion is elongated in an oblique direction, indicating that the organ motion dosimetric modeling using Gaussian kernel may need to be modified to account for the particular organ motion character of prostate.

Dataset Information

Real time ligand-induced motion mappings of AChBP and nAChR using X-ray single molecule tracking.

Publications

Real time ligand-induced motion mappings of AChBP and nAChR using X-ray single molecule tracking.

Similar Datasets

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