Real time ligand-induced motion mappings of AChBP and nAChR using X-ray single molecule tracking.
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ABSTRACT:
SUBMITTER: Sekiguchi H
PROVIDER: S-EPMC4165275 | biostudies-literature | 2014 Sep
REPOSITORIES: biostudies-literature
Publications
Real time ligand-induced motion mappings of AChBP and nAChR using X-ray single molecule tracking.
Sekiguchi Hiroshi H Suzuki Yasuhito Y Nishino Yuri Y Kobayashi Suzuko S Shimoyama Yoshiko Y Cai Weiyan W Nagata Kenji K Okada Masato M Ichiyanagi Kouhei K Ohta Noboru N Yagi Naoto N Miyazawa Atsuo A Kubo Tai T Sasaki Yuji C YC
Scientific reports 20140916
We observed the dynamic three-dimensional (3D) single molecule behaviour of acetylcholine-binding protein (AChBP) and nicotinic acetylcholine receptor (nAChR) using a single molecule tracking technique, diffracted X-ray tracking (DXT) with atomic scale and 100 μs time resolution. We found that the combined tilting and twisting motions of the proteins were enhanced upon acetylcholine (ACh) binding. We present the internal motion maps of AChBP and nAChR in the presence of either ACh or α-bungaroto ...[more]
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