Ontology highlight
ABSTRACT:
SUBMITTER: Stanton BC
PROVIDER: S-EPMC4165527 | biostudies-literature | 2014 Feb
REPOSITORIES: biostudies-literature
Stanton Brynne C BC Nielsen Alec A K AA Tamsir Alvin A Clancy Kevin K Peterson Todd T Voigt Christopher A CA
Nature chemical biology 20131208 2
Genetic circuits perform computational operations based on interactions between freely diffusing molecules within a cell. When transcription factors are combined to build a circuit, unintended interactions can disrupt its function. Here, we apply 'part mining' to build a library of 73 TetR-family repressors gleaned from prokaryotic genomes. The operators of a subset were determined using an in vitro method, and this information was used to build synthetic promoters. The promoters and repressors ...[more]