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Principles for applying optogenetic tools derived from direct comparative analysis of microbial opsins.


ABSTRACT: Diverse optogenetic tools have allowed versatile control over neural activity. Many depolarizing and hyperpolarizing tools have now been developed in multiple laboratories and tested across different preparations, presenting opportunities but also making it difficult to draw direct comparisons. This challenge has been compounded by the dependence of performance on parameters such as vector, promoter, expression time, illumination, cell type and many other variables. As a result, it has become increasingly complicated for end users to select the optimal reagents for their experimental needs. For a rapidly growing field, critical figures of merit should be formalized both to establish a framework for further development and so that end users can readily understand how these standardized parameters translate into performance. Here we systematically compared microbial opsins under matched experimental conditions to extract essential principles and identify key parameters for the conduct, design and interpretation of experiments involving optogenetic techniques.

SUBMITTER: Mattis J 

PROVIDER: S-EPMC4165888 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature

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Principles for applying optogenetic tools derived from direct comparative analysis of microbial opsins.

Mattis Joanna J   Tye Kay M KM   Ferenczi Emily A EA   Ramakrishnan Charu C   O'Shea Daniel J DJ   Prakash Rohit R   Gunaydin Lisa A LA   Hyun Minsuk M   Fenno Lief E LE   Gradinaru Viviana V   Yizhar Ofer O   Deisseroth Karl K  

Nature methods 20111218 2


Diverse optogenetic tools have allowed versatile control over neural activity. Many depolarizing and hyperpolarizing tools have now been developed in multiple laboratories and tested across different preparations, presenting opportunities but also making it difficult to draw direct comparisons. This challenge has been compounded by the dependence of performance on parameters such as vector, promoter, expression time, illumination, cell type and many other variables. As a result, it has become in  ...[more]

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