Ontology highlight
ABSTRACT:
SUBMITTER: Yen ST
PROVIDER: S-EPMC4166609 | biostudies-literature | 2014 Sep
REPOSITORIES: biostudies-literature
Yen Shuo-Ting ST Zhang Min M Deng Jian Min JM Usman Shireen J SJ Smith Chad N CN Parker-Thornburg Jan J Swinton Paul G PG Martin James F JF Behringer Richard R RR
Developmental biology 20140628 1
Tyrosinase is the rate-limiting enzyme for the production of melanin pigmentation. In the mouse and other animals, homozygous null mutations in the Tyrosinase gene (Tyr) result in the absence of pigmentation, i.e. albinism. Here we used the CRISPR/Cas9 system to generate mono- and bi-allelic null mutations in the Tyr locus by zygote injection of two single-guide and Cas9 RNAs. Injection into C57BL/6N wild-type embryos resulted in one completely albino founder carrying two different Tyr mutations ...[more]