DNA methylation increases nucleosome compaction and rigidity.
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ABSTRACT:
SUBMITTER: Choy JS
PROVIDER: S-EPMC4167393 | biostudies-literature | 2010 Feb
REPOSITORIES: biostudies-literature
Publications
DNA methylation increases nucleosome compaction and rigidity.
Choy John S JS Wei Sijie S Lee Ju Yeon JY Tan Song S Chu Steven S Lee Tae-Hee TH
Journal of the American Chemical Society 20100201 6
Cytosine methylation on CpG dinucleotides is an essential epigenetic modification in eukaryotes. How DNA methylation modulates nucleosome structure and dynamics has been a long-standing question. We implemented a single-molecule method to monitor the effects of DNA methylation on the structure and dynamics of mononucleosomes. Our studies show that DNA methylation induces a more compact and rigid nucleosome structure, providing a physical basis for how DNA methylation might contribute to regulati ...[more]
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