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Gene expression profiling of biological pathway alterations by radiation exposure.


ABSTRACT: Though damage caused by radiation has been the focus of rigorous research, the mechanisms through which radiation exerts harmful effects on cells are complex and not well-understood. In particular, the influence of low dose radiation exposure on the regulation of genes and pathways remains unclear. In an attempt to investigate the molecular alterations induced by varying doses of radiation, a genome-wide expression analysis was conducted. Peripheral blood mononuclear cells were collected from five participants and each sample was subjected to 0.5 Gy, 1 Gy, 2.5 Gy, and 5 Gy of cobalt 60 radiation, followed by array-based expression profiling. Gene set enrichment analysis indicated that the immune system and cancer development pathways appeared to be the major affected targets by radiation exposure. Therefore, 1 Gy radioactive exposure seemed to be a critical threshold dosage. In fact, after 1 Gy radiation exposure, expression levels of several genes including FADD, TNFRSF10B, TNFRSF8, TNFRSF10A, TNFSF10, TNFSF8, CASP1, and CASP4 that are associated with carcinogenesis and metabolic disorders showed significant alterations. Our results suggest that exposure to low-dose radiation may elicit changes in metabolic and immune pathways, potentially increasing the risk of immune dysfunctions and metabolic disorders.

SUBMITTER: Lee KF 

PROVIDER: S-EPMC4170887 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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Gene expression profiling of biological pathway alterations by radiation exposure.

Lee Kuei-Fang KF   Weng Julia Tzu-Ya JT   Hsu Paul Wei-Che PW   Chi Yu-Hsiang YH   Chen Ching-Kai CK   Liu Ingrid Y IY   Chen Yi-Cheng YC   Wu Lawrence Shih-Hsin LS  

BioMed research international 20140908


Though damage caused by radiation has been the focus of rigorous research, the mechanisms through which radiation exerts harmful effects on cells are complex and not well-understood. In particular, the influence of low dose radiation exposure on the regulation of genes and pathways remains unclear. In an attempt to investigate the molecular alterations induced by varying doses of radiation, a genome-wide expression analysis was conducted. Peripheral blood mononuclear cells were collected from fi  ...[more]

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