Targeted genomic rearrangements using CRISPR/Cas technology.
Ontology highlight
ABSTRACT:
SUBMITTER: Choi PS
PROVIDER: S-EPMC4170920 | biostudies-literature | 2014 Apr
REPOSITORIES: biostudies-literature
Publications
Targeted genomic rearrangements using CRISPR/Cas technology.
Choi Peter S PS Meyerson Matthew M
Nature communications 20140424
Genomic rearrangements are frequently observed in cancer cells but have been difficult to generate in a highly specific manner for functional analysis. Here we report the application of CRISPR/Cas technology to successfully generate several types of chromosomal rearrangements implicated as driver events in lung cancer, including the CD74-ROS1 translocation event and the EML4-ALK and KIF5B-RET inversion events. Our results demonstrate that Cas9-induced DNA breaks promote efficient rearrangement b ...[more]
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