Transcriptional profiling of cells sorted by RNA abundance.
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ABSTRACT:
SUBMITTER: Klemm S
PROVIDER: S-EPMC4174458 | biostudies-literature | 2014 May
REPOSITORIES: biostudies-literature
Publications
Transcriptional profiling of cells sorted by RNA abundance.
Klemm Sandy S Semrau Stefan S Wiebrands Kay K Mooijman Dylan D Faddah Dina A DA Jaenisch Rudolf R van Oudenaarden Alexander A
Nature methods 20140330 5
We have developed a quantitative technique for sorting cells on the basis of endogenous RNA abundance, with a molecular resolution of 10-20 transcripts. We demonstrate efficient and unbiased RNA extraction from transcriptionally sorted cells and report a high-fidelity transcriptome measurement of mouse induced pluripotent stem cells (iPSCs) isolated from a heterogeneous reprogramming culture. This method is broadly applicable to profiling transcriptionally distinct cellular states without requir ...[more]
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