Ontology highlight
ABSTRACT:
SUBMITTER: Strande NT
PROVIDER: S-EPMC4176175 | biostudies-literature | 2014
REPOSITORIES: biostudies-literature
Strande Natasha T NT Carvajal-Garcia Juan J Hallett Ryan A RA Waters Crystal A CA Roberts Steven A SA Strom Christina C Kuhlman Brian B Ramsden Dale A DA
Nucleic acids research 20140908 17
The non-homologous end joining (NHEJ) pathway is used in diverse species to repair chromosome breaks, and is defined in part by a requirement for Ku. We previously demonstrated mammalian Ku has intrinsic 5' deoxyribosephosphate (5'dRP) and apurinic/apyrimidinic (AP) lyase activity, and showed this activity is important for excising abasic site damage from ends. Here we employ systematic mutagenesis to clarify the protein requirements for this activity. We identify lysine 31 in the 70 kD subunit ...[more]