Ontology highlight
ABSTRACT:
SUBMITTER: Jha JK
PROVIDER: S-EPMC4176361 | biostudies-literature | 2014
REPOSITORIES: biostudies-literature
Jha Jyoti K JK Ghirlando Rodolfo R Chattoraj Dhruba K DK
Nucleic acids research 20140826 16
RctB, the initiator of replication of Vibrio cholerae chromosome 2 (chr2), binds to the origin of replication to specific 12-mer sites both as a monomer and a dimer. Binding to 12-mers is essential for initiation. The monomers also bind to a second kind of site, 39-mers, which inhibits initiation. Mutations in rctB that reduce dimer binding increase monomer binding to 12-mers but decrease monomer binding to 39-mers. The mechanism of this paradoxical binding behavior has been unclear. Using delet ...[more]