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Oligomerisation of C. elegans olfactory receptors, ODR-10 and STR-112, in yeast.

ABSTRACT: It is widely accepted that vertebrate G-Protein Coupled Receptors (GPCRs) associate with each other as homo- or hetero-dimers or higher-order oligomers. The C. elegans genome encodes hundreds of olfactory GPCRs, which may be expressed in fewer than a dozen chemosensory neurons, suggesting an opportunity for oligomerisation. Here we show, using three independent lines of evidence: co-immunoprecipitation, bioluminescence resonance energy transfer and a yeast two-hybrid assay that nematode olfactory receptors (ORs) oligomerise when heterologously expressed in yeast. Specifically, the nematode receptor ODR-10 is able to homo-oligomerise and can also form heteromers with the related nematode receptor STR-112. ODR-10 also oligomerised with the rat I7 OR but did not oligomerise with the human somatostatin receptor 5, a neuropeptide receptor. In this study, the question of functional relevance was not addressed and remains to be investigated.

SUBMITTER: Tehseen M

PROVIDER: S-EPMC4177895 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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Oligomerisation of C. elegans olfactory receptors, ODR-10 and STR-112, in yeast.

Tehseen Muhammad M Liao Chunyan C Dacres Helen H Dumancic Mira M Trowell Stephen S Anderson Alisha A

PloS one 20140925 9

It is widely accepted that vertebrate G-Protein Coupled Receptors (GPCRs) associate with each other as homo- or hetero-dimers or higher-order oligomers. The C. elegans genome encodes hundreds of olfactory GPCRs, which may be expressed in fewer than a dozen chemosensory neurons, suggesting an opportunity for oligomerisation. Here we show, using three independent lines of evidence: co-immunoprecipitation, bioluminescence resonance energy transfer and a yeast two-hybrid assay that nematode olfactor ...[more]

PMID: 25254556

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