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Molecular characterization of three Lactobacillus delbrueckii subsp. bulgaricus phages.


ABSTRACT: In this study, three phages infecting Lactobacillus delbrueckii subsp. bulgaricus, named Ld3, Ld17, and Ld25A, were isolated from whey samples obtained from various industrial fermentations. These phages were further characterized in a multifaceted approach: (i) biological and physical characterization through host range analysis and electron microscopy; (ii) genetic assessment through genome analysis; (iii) mass spectrometry analysis of the structural components of the phages; and (iv), for one phage, transcriptional analysis by Northern hybridization, reverse transcription-PCR, and primer extension. The three obtained phage genomes display high levels of sequence identity to each other and to genomes of the so-called group b L. delbrueckii phages c5, LL-Ku, and phiLdb, where some of the observed differences are believed to be responsible for host range variations.

SUBMITTER: Casey E 

PROVIDER: S-EPMC4178594 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature

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Molecular characterization of three Lactobacillus delbrueckii subsp. bulgaricus phages.

Casey Eoghan E   Mahony Jennifer J   O'Connell-Motherway Mary M   Bottacini Francesca F   Cornelissen Anneleen A   Neve Horst H   Heller Knut J KJ   Noben Jean-Paul JP   Dal Bello Fabio F   van Sinderen Douwe D  

Applied and environmental microbiology 20140707 18


In this study, three phages infecting Lactobacillus delbrueckii subsp. bulgaricus, named Ld3, Ld17, and Ld25A, were isolated from whey samples obtained from various industrial fermentations. These phages were further characterized in a multifaceted approach: (i) biological and physical characterization through host range analysis and electron microscopy; (ii) genetic assessment through genome analysis; (iii) mass spectrometry analysis of the structural components of the phages; and (iv), for one  ...[more]

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