Dataset Information

Different effects of transgenic maize and nontransgenic maize on nitrogen-transforming archaea and bacteria in tropical soils.

ABSTRACT: The composition of the rhizosphere microbiome is a result of interactions between plant roots, soil, and environmental conditions. The impact of genetic variation in plant species on the composition of the root-associated microbiota remains poorly understood. This study assessed the abundances and structures of nitrogen-transforming (ammonia-oxidizing) archaea and bacteria as well as nitrogen-fixing bacteria driven by genetic modification of their maize host plants. The data show that significant changes in the abundances (revealed by quantitative PCR) of ammonia-oxidizing bacterial and archaeal communities occurred as a result of the maize host being genetically modified. In contrast, the structures of the total communities (determined by PCR-denaturing gradient gel electrophoresis) were mainly driven by factors such as soil type and season and not by plant genotype. Thus, the abundances of ammonia-oxidizing bacterial and archaeal communities but not structures of those communities were revealed to be responsive to changes in maize genotype, allowing the suggestion that community abundances should be explored as candidate bioindicators for monitoring the possible impacts of cultivation of genetically modified plants.

SUBMITTER: Cotta SR

PROVIDER: S-EPMC4178641 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Different effects of transgenic maize and nontransgenic maize on nitrogen-transforming archaea and bacteria in tropical soils.

Cotta Simone Raposo SR Dias Armando Cavalcante Franco AC Marriel Ivanildo Evódio IE Andreote Fernando Dini FD Seldin Lucy L van Elsas Jan Dirk JD

Applied and environmental microbiology 20140808 20

The composition of the rhizosphere microbiome is a result of interactions between plant roots, soil, and environmental conditions. The impact of genetic variation in plant species on the composition of the root-associated microbiota remains poorly understood. This study assessed the abundances and structures of nitrogen-transforming (ammonia-oxidizing) archaea and bacteria as well as nitrogen-fixing bacteria driven by genetic modification of their maize host plants. The data show that significan ...[more]

PMID: 25107970

Similar Datasets

Project description:Non-microbial methane (NM-CH4), emissions from soil might play a significant role in carbon cycling and global climate change. However, the production mechanisms and emission potential of soil NM-CH4 from tropical rainforest remain highly uncertain. In order to explore the laws and characteristics of NM-CH4 emission from tropical rainforest soils. Incubation experiments at different environmental conditions (temperatures, soil water contents, hydrogen peroxide) and for soils with different soil organic carbon (SOC) contents were conducted to investigate the NM-CH4 emission characteristics and its influence factors of soils (0-10cm) that collected from a tropical rainforest in Hainan, China. Incubation results illustrated that soil NM-CH4 release showed a linear increase with the incubation time in the first 24 hours at 70 °C, whereas the logarithmic curve increase was found in 192 h incubation. Soil NM-CH4 emission rates under aerobic condition were significantly higher than that of under anaerobic condition at first 24 h incubation. The increasing of temperature, suitable soil water contents (0-100%), and hydrogen peroxide significantly promoted soil NM-CH4 emission rates at the first 24 h incubation. However, excessive soil water contents (200%) inhibited soil NM-CH4 emissions. According to the curve simulated from the NM-CH4 emission rates and incubation time at 70 °C of aerobic condition, soil would no longer release NM-CH4 after 229 h incubation. The NM-CH4 emissions were positively corelated with SOC contents, and the average soil NM-CH4 emission potential was about 6.91 ug per gram organic carbon in the tropical mountain rainforest. This study revealed that soils in the tropical rainforest could produce NM-CH4 under certain environment conditions and it supported production mechanisms of thermal degradation and reactive oxygen species oxidation. Those results could provide a basic data for understanding the soil NM-CH4 production mechanisms and its potential in the tropical rainforest.

Dataset Information

Different effects of transgenic maize and nontransgenic maize on nitrogen-transforming archaea and bacteria in tropical soils.

Publications

Different effects of transgenic maize and nontransgenic maize on nitrogen-transforming archaea and bacteria in tropical soils.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets