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A substitution in the transmembrane region of the glycoprotein leads to an unstable attenuation of Machupo virus.

ABSTRACT: Machupo virus (MACV) is the etiologic agent of Bolivian hemorrhagic fever (BHF). Utilizing a reverse-genetics system recently developed, we report the rescue of a rationally modified recombinant MACV containing a single mutation in the transmembrane region of the glycoprotein. Following challenge of susceptible mice, we identified a significant reduction in virulence in the novel virus. We also identified an instability leading to reversion of the single mutation to a wild-type genotype.

SUBMITTER: Patterson M

PROVIDER: S-EPMC4178861 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature

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A substitution in the transmembrane region of the glycoprotein leads to an unstable attenuation of Machupo virus.

Patterson Michael M Koma Takaaki T Seregin Alexey A Huang Cheng C Miller Milagros M Smith Jennifer J Yun Nadezhda N Smith Jeanon J Paessler Slobodan S

Journal of virology 20140716 18

Machupo virus (MACV) is the etiologic agent of Bolivian hemorrhagic fever (BHF). Utilizing a reverse-genetics system recently developed, we report the rescue of a rationally modified recombinant MACV containing a single mutation in the transmembrane region of the glycoprotein. Following challenge of susceptible mice, we identified a significant reduction in virulence in the novel virus. We also identified an instability leading to reversion of the single mutation to a wild-type genotype. ...[more]

PMID: 25031335

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