Recent H3N2 influenza virus clinical isolates rapidly acquire hemagglutinin or neuraminidase mutations when propagated for antigenic analyses.
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ABSTRACT:
SUBMITTER: Chambers BS
PROVIDER: S-EPMC4178893 | biostudies-literature | 2014 Sep
REPOSITORIES: biostudies-literature
Publications
Recent H3N2 influenza virus clinical isolates rapidly acquire hemagglutinin or neuraminidase mutations when propagated for antigenic analyses.
Chambers Benjamin S BS Li Yang Y Hodinka Richard L RL Hensley Scott E SE
Journal of virology 20140702 18
Prior to serological testing, influenza viruses are typically propagated in eggs or cell culture. Recent human H3N2 strains bind to cells with low avidity. Here, we isolated nine primary H3N2 viral isolates from respiratory secretions of children. Upon propagation in vitro, five of these isolates acquired hemagglutinin or neuraminidase mutations that increased virus binding to cell surfaces. These mutations can potentially confound serological assays commonly used to identify antigenically novel ...[more]
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