Dataset Information

Global transcriptome analysis and identification of the flowering regulatory genes expressed in leaves of Lagerstroemia indica.

ABSTRACT: Flowering time is an important trait for ornamental plants, and flowering regulation has thus been both a focus of and challenge to researchers. Lagerstroemia indica is an important summer flowering tree in China and has been introduced abroad as a key parent of new cultivars; no previous reports have addressed the regulation of flowering time in this species. In this study, 28,567,778×2 reads were obtained from leaves of L. indica. A total of 37,325 unigenes were assembled with an average length of 849.56?bp, and 17,506 (46.90%) unigenes were significantly matched to known genes in the nr database of GenBank. The annotated sequences were clustered into putative functional categories using the Gene Ontology framework. Potential genes and their functions were predicted by the Cluster of Orthologous Groups analysis and Kyoto Encyclopedia of Genes and Genomes pathway mapping. A total of 115 unigenes related to flowering time control were discovered. Ten homologous genes of the CONSTANS-like (COL) gene family were identified based on transcript data. Phylogenetic analysis of the CONSTANS and COL genes from L. indica and other species grouped them into three clades. The transcriptome dataset and outcome of the analysis provide a valuable new resource for research on the functional genomics and molecular mechanisms of flowering control in L. indica.

SUBMITTER: Zhang Z

PROVIDER: S-EPMC4179922 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature

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Global transcriptome analysis and identification of the flowering regulatory genes expressed in leaves of Lagerstroemia indica.

Zhang Zhenyu Z Wang Peng P Li Ya Y Ma Lingling L Li Linfang L Yang Rutong R Ma Yuzhu Y Wang Shu'an S Wang Qing Q

DNA and cell biology 20140701 10

Flowering time is an important trait for ornamental plants, and flowering regulation has thus been both a focus of and challenge to researchers. Lagerstroemia indica is an important summer flowering tree in China and has been introduced abroad as a key parent of new cultivars; no previous reports have addressed the regulation of flowering time in this species. In this study, 28,567,778×2 reads were obtained from leaves of L. indica. A total of 37,325 unigenes were assembled with an average lengt ...[more]

PMID: 24983664

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Project description:BackgroundAlfalfa (Medicago sativa L.) is a perennial legume extensively planted throughout the world as a high nutritive value livestock forage. Flowering time is an important agronomic trait that contributes to the production of alfalfa hay and seeds. However, the underlying molecular mechanisms of flowering time regulation in alfalfa are not well understood.ResultsIn this study, an early-flowering alfalfa genotype 80 and a late-flowering alfalfa genotype 195 were characterized for the flowering phenotype. Our analysis revealed that the lower jasmonate (JA) content in new leaves and the downregulation of JA biosynthetic genes (i.e. lipoxygenase, the 12-oxophytodienoate reductase-like protein, and salicylic acid carboxyl methyltransferase) may play essential roles in the early-flowering phenotype of genotype 80. Further research indicated that genes encode pathogenesis-related proteins [e.g. leucine rich repeat (LRR) family proteins, receptor-like proteins, and toll-interleukin-like receptor (TIR)-nucleotide-binding site (NBS)-LRR class proteins] and members of the signaling receptor kinase family [LRR proteins, kinases domain of unknown function 26 (DUF26) and wheat leucine-rich repeat receptor-like kinase10 (LRK10)-like kinases] are related to early flowering in alfalfa. Additionally, those involved in secondary metabolism (2-oxoglutarate/Fe (II)-dependent dioxygenases and UDP-glycosyltransferase) and the proteasome degradation pathway [really interesting new gene (RING)/U-box superfamily proteins and F-box family proteins] are also related to early flowering in alfalfa.ConclusionsIntegrated phenotypical, physiological, and transcriptomic analyses demonstrate that hormone biosynthesis and signaling pathways, pathogenesis-related genes, signaling receptor kinase family genes, secondary metabolism genes, and proteasome degradation pathway genes are responsible for the early flowering phenotype in alfalfa. This will provide new insights into future studies of flowering time in alfalfa and inform genetic improvement strategies for optimizing this important trait.

Dataset Information

Global transcriptome analysis and identification of the flowering regulatory genes expressed in leaves of Lagerstroemia indica.

Publications

Global transcriptome analysis and identification of the flowering regulatory genes expressed in leaves of Lagerstroemia indica.

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