Cyclophilin D modulates mitochondrial acetylome.

ABSTRACT:

Rationale

Mice lacking cyclophilin D (CypD(-/-)), a mitochondrial chaperone protein, have altered cardiac metabolism. As acetylation has been shown to regulate metabolism, we tested whether changes in protein acetylation might play a role in these metabolic changes in CypD(-/-) hearts.

Objective

Our aim was to test the hypothesis that loss of CypD alters the cardiac mitochondrial acetylome.

Methods and results

To identify changes in lysine-acetylated proteins and to map acetylation sites after ablation of CypD, we subjected tryptic digests of isolated cardiac mitochondria from wild-type and CypD(-/-) mice to immunoprecipitation using agarose beads coupled to antiacetyl lysine antibodies followed by mass spectrometry. We used label-free analysis for the relative quanti

SUBMITTER: Nguyen TT 

PROVIDER: S-EPMC4180423 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature