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Far-red organic fluorophores contain a fluorescent impurity.

ABSTRACT: Far-red organic fluorophores commonly used in traditional and super-resolution localization microscopy are found to contain a fluorescent impurity with green excitation and near-red emission. This near-red fluorescent impurity can interfere with some multicolor stochastic optical reconstruction microscopy/photoactivated localization microscopy measurements in live cells and produce subtle artifacts in chemically fixed cells. We additionally describe alternatives to avoid artifacts in super-resolution localization microscopy.

SUBMITTER: Stone MB

PROVIDER: S-EPMC4180537 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature

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Far-red organic fluorophores contain a fluorescent impurity.

Stone Matthew B MB Veatch Sarah L SL

Chemphyschem : a European journal of chemical physics and physical chemistry 20140429 11

Far-red organic fluorophores commonly used in traditional and super-resolution localization microscopy are found to contain a fluorescent impurity with green excitation and near-red emission. This near-red fluorescent impurity can interfere with some multicolor stochastic optical reconstruction microscopy/photoactivated localization microscopy measurements in live cells and produce subtle artifacts in chemically fixed cells. We additionally describe alternatives to avoid artifacts in super-resol ...[more]

PMID: 24782148

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