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Bioengineered functional brain-like cortical tissue.

ABSTRACT: The brain remains one of the most important but least understood tissues in our body, in part because of its complexity as well as the limitations associated with in vivo studies. Although simpler tissues have yielded to the emerging tools for in vitro 3D tissue cultures, functional brain-like tissues have not. We report the construction of complex functional 3D brain-like cortical tissue, maintained for months in vitro, formed from primary cortical neurons in modular 3D compartmentalized architectures with electrophysiological function. We show that, on injury, this brain-like tissue responds in vitro with biochemical and electrophysiological outcomes that mimic observations in vivo. This modular 3D brain-like tissue is capable of real-time nondestructive assessments, offering previously unidentified directions for studies of brain homeostasis and injury.

SUBMITTER: Tang-Schomer MD

PROVIDER: S-EPMC4183301 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature

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Bioengineered functional brain-like cortical tissue.

Tang-Schomer Min D MD White James D JD Tien Lee W LW Schmitt L Ian LI Valentin Thomas M TM Graziano Daniel J DJ Hopkins Amy M AM Omenetto Fiorenzo G FG Haydon Philip G PG Kaplan David L DL

Proceedings of the National Academy of Sciences of the United States of America 20140811 38

The brain remains one of the most important but least understood tissues in our body, in part because of its complexity as well as the limitations associated with in vivo studies. Although simpler tissues have yielded to the emerging tools for in vitro 3D tissue cultures, functional brain-like tissues have not. We report the construction of complex functional 3D brain-like cortical tissue, maintained for months in vitro, formed from primary cortical neurons in modular 3D compartmentalized archit ...[more]

PMID: 25114234

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