Dataset Information

CDCP1 identifies a CD146 negative subset of marrow fibroblasts involved with cytokine production.

ABSTRACT: In vitro expanded bone marrow stromal cells contain at least two populations of fibroblasts, a CD146/MCAM positive population, previously reported to be critical for establishing the stem cell niche and a CD146-negative population that expresses CUB domain-containing protein 1 (CDCP1)/CD318. Immunohistochemistry of marrow biopsies shows that clusters of CDCP1+ cells are present in discrete areas distinct from areas of fibroblasts expressing CD146. Using a stromal cell line, HS5, which approximates primary CDCP1+ stromal cells, we show that binding of an activating antibody against CDCP1 results in tyrosine-phosphorylation of CDCP1, paralleled by phosphorylation of Src Family Kinases (SFKs) Protein Kinase C delta (PKC-?). When CDCP1 expression is knocked-down by siRNA, the expression and secretion of myelopoietic cytokines is increased. These data suggest CDCP1 expression can be used to identify a subset of marrow fibroblasts functionally distinct from CD146+ fibroblasts. Furthermore the CDCP1 protein may contribute to the defining function of these cells by regulating cytokine expression.

SUBMITTER: Iwata M

PROVIDER: S-EPMC4183599 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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CDCP1 identifies a CD146 negative subset of marrow fibroblasts involved with cytokine production.

Iwata Mineo M Torok-Storb Beverly B Wayner Elizabeth A EA Carter William G WG

PloS one 20141002 10

In vitro expanded bone marrow stromal cells contain at least two populations of fibroblasts, a CD146/MCAM positive population, previously reported to be critical for establishing the stem cell niche and a CD146-negative population that expresses CUB domain-containing protein 1 (CDCP1)/CD318. Immunohistochemistry of marrow biopsies shows that clusters of CDCP1+ cells are present in discrete areas distinct from areas of fibroblasts expressing CD146. Using a stromal cell line, HS5, which approximat ...[more]

PMID: 25275584

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Dataset Information

CDCP1 identifies a CD146 negative subset of marrow fibroblasts involved with cytokine production.

Publications

CDCP1 identifies a CD146 negative subset of marrow fibroblasts involved with cytokine production.

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