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Heterobifunctional PEG ligands for bioconjugation reactions on iron oxide nanoparticles.


ABSTRACT: Ever since iron oxide nanoparticles have been recognized as promising scaffolds for biomedical applications, their surface functionalization has become even more important. We report the synthesis of a novel polyethylene glycol-based ligand that combines multiple advantageous properties for these applications. The ligand is covalently bound to the surface via a siloxane group, while its polyethylene glycol backbone significantly improves the colloidal stability of the particle in complex environments. End-capping the molecule with a carboxylic acid introduces a variety of coupling chemistry possibilities. In this study an antibody targeting plasminogen activator inhibitor-1 was coupled to the surface and its presence and binding activity was assessed by enzyme-linked immunosorbent assay and surface plasmon resonance experiments. The results indicate that the ligand has high potential towards biomedical applications where colloidal stability and advanced functionality is crucial.

SUBMITTER: Bloemen M 

PROVIDER: S-EPMC4183648 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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Heterobifunctional PEG ligands for bioconjugation reactions on iron oxide nanoparticles.

Bloemen Maarten M   Van Stappen Thomas T   Willot Pieter P   Lammertyn Jeroen J   Koeckelberghs Guy G   Geukens Nick N   Gils Ann A   Verbiest Thierry T  

PloS one 20141002 10


Ever since iron oxide nanoparticles have been recognized as promising scaffolds for biomedical applications, their surface functionalization has become even more important. We report the synthesis of a novel polyethylene glycol-based ligand that combines multiple advantageous properties for these applications. The ligand is covalently bound to the surface via a siloxane group, while its polyethylene glycol backbone significantly improves the colloidal stability of the particle in complex environ  ...[more]

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