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Formation of amyloid fibers by monomeric light chain variable domains.

ABSTRACT: Systemic light chain amyloidosis is a lethal disease characterized by excess immunoglobulin light chains and light chain fragments composed of variable domains, which aggregate into amyloid fibers. These fibers accumulate and damage organs. Some light chains induce formation of amyloid fibers, whereas others do not, making it unclear what distinguishes amyloid formers from non-formers. One mechanism by which sequence variation may reduce propensity to form amyloid fibers is by shifting the equilibrium toward an amyloid-resistant quaternary structure. Here we identify the monomeric form of the Mcg immunoglobulin light chain variable domain as the quaternary unit required for amyloid fiber assembly. Dimers of Mcg variable domains remain stable and soluble, yet become prone to assemble into amyloid fibers upon disassociation into monomers.

SUBMITTER: Brumshtein B

PROVIDER: S-EPMC4183792 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature

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Formation of amyloid fibers by monomeric light chain variable domains.

Brumshtein Boris B Esswein Shannon R SR Landau Meytal M Ryan Christopher M CM Whitelegge Julian P JP Phillips Martin L ML Cascio Duilio D Sawaya Michael R MR Eisenberg David S DS

The Journal of biological chemistry 20140819 40

Systemic light chain amyloidosis is a lethal disease characterized by excess immunoglobulin light chains and light chain fragments composed of variable domains, which aggregate into amyloid fibers. These fibers accumulate and damage organs. Some light chains induce formation of amyloid fibers, whereas others do not, making it unclear what distinguishes amyloid formers from non-formers. One mechanism by which sequence variation may reduce propensity to form amyloid fibers is by shifting the equil ...[more]

PMID: 25138218

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