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Isolation and molecular characterization of contagious pustular dermatitis virus from Rajasthan, India.

ABSTRACT: This article describes the isolation and identification of contagious pustular dermatitis virus/orf virus (ORFV) from an outbreak of contagious pustular dermatitis (orf) in flocks of goats, in the north western region of India (Rajasthan). The virus was isolated in Vero cell cultures from scab and swab suspensions and has been identified using GIF/IL-2 and B2L gene specific primers in PCR and sequencing. The virus showed high nucleotide identity with previously reported Chinese, far eastern, Brazilian and Indian isolates. This report described the use of molecular tools for fast, reliable and confirmatory diagnosis of ORFV infection.

SUBMITTER: Maan S

PROVIDER: S-EPMC4188203 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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Isolation and molecular characterization of contagious pustular dermatitis virus from Rajasthan, India.

Maan Sushila S Kumar Aman A Batra Kanisht K Singh Mahendra M Nanda Trilok T Ghosh Arnab A Maan Narender S NS

Virusdisease 20140301 3

This article describes the isolation and identification of contagious pustular dermatitis virus/orf virus (ORFV) from an outbreak of contagious pustular dermatitis (orf) in flocks of goats, in the north western region of India (Rajasthan). The virus was isolated in Vero cell cultures from scab and swab suspensions and has been identified using GIF/IL-2 and B2L gene specific primers in PCR and sequencing. The virus showed high nucleotide identity with previously reported Chinese, far eastern, Bra ...[more]

PMID: 25674607

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