Dataset Information

Unraveling the sequence-dependent polymorphic behavior of d(CpG) steps in B-DNA.

ABSTRACT: We have made a detailed study of one of the most surprising sources of polymorphism in B-DNA: the high twist/low twist (HT/LT) conformational change in the d(CpG) base pair step. Using extensive computations, complemented with database analysis, we were able to characterize the twist polymorphism in the d(CpG) step in all the possible tetranucleotide environment. We found that twist polymorphism is coupled with BI/BII transitions, and, quite surprisingly, with slide polymorphism in the neighboring step. Unexpectedly, the penetration of cations into the minor groove of the d(CpG) step seems to be the key element in promoting twist transitions. The tetranucleotide environment also plays an important role in the sequence-dependent d(CpG) polymorphism. In this connection, we have detected a previously unexplored intramolecular C-H···O hydrogen bond interaction that stabilizes the low twist state when 3'-purines flank the d(CpG) step. This work explains a coupled mechanism involving several apparently uncorrelated conformational transitions that has only been partially inferred by earlier experimental or theoretical studies. Our results provide a complete description of twist polymorphism in d(CpG) steps and a detailed picture of the molecular choreography associated with this conformational change.

SUBMITTER: Dans PD

PROVIDER: S-EPMC4191396 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature

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Unraveling the sequence-dependent polymorphic behavior of d(CpG) steps in B-DNA.

Dans Pablo Daniel PD Faustino Ignacio I Battistini Federica F Zakrzewska Krystyna K Lavery Richard R Orozco Modesto M

Nucleic acids research 20140915 18

We have made a detailed study of one of the most surprising sources of polymorphism in B-DNA: the high twist/low twist (HT/LT) conformational change in the d(CpG) base pair step. Using extensive computations, complemented with database analysis, we were able to characterize the twist polymorphism in the d(CpG) step in all the possible tetranucleotide environment. We found that twist polymorphism is coupled with BI/BII transitions, and, quite surprisingly, with slide polymorphism in the neighbori ...[more]

PMID: 25223784

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Dataset Information

Unraveling the sequence-dependent polymorphic behavior of d(CpG) steps in B-DNA.

Publications

Unraveling the sequence-dependent polymorphic behavior of d(CpG) steps in B-DNA.

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