Ontology highlight
ABSTRACT:
SUBMITTER: Pollen AA
PROVIDER: S-EPMC4191988 | biostudies-literature | 2014 Oct
REPOSITORIES: biostudies-literature
Pollen Alex A AA Nowakowski Tomasz J TJ Shuga Joe J Wang Xiaohui X Leyrat Anne A AA Lui Jan H JH Li Nianzhen N Szpankowski Lukasz L Fowler Brian B Chen Peilin P Ramalingam Naveen N Sun Gang G Thu Myo M Norris Michael M Lebofsky Ronald R Toppani Dominique D Kemp Darnell W DW Wong Michael M Clerkson Barry B Jones Brittnee N BN Wu Shiquan S Knutsson Lawrence L Alvarado Beatriz B Wang Jing J Weaver Lesley S LS May Andrew P AP Jones Robert C RC Unger Marc A MA Kriegstein Arnold R AR West Jay A A JA
Nature biotechnology 20140803 10
Large-scale surveys of single-cell gene expression have the potential to reveal rare cell populations and lineage relationships but require efficient methods for cell capture and mRNA sequencing. Although cellular barcoding strategies allow parallel sequencing of single cells at ultra-low depths, the limitations of shallow sequencing have not been investigated directly. By capturing 301 single cells from 11 populations using microfluidics and analyzing single-cell transcriptomes across downsampl ...[more]