Unknown

Dataset Information

0

Polo-like kinase 1 licenses CENP-A deposition at centromeres.


ABSTRACT: To ensure the stable transmission of the genome during vertebrate cell division, the mitotic spindle must attach to a single locus on each chromosome, termed the centromere. The fundamental requirement for faithful centromere inheritance is the controlled deposition of the centromere-specifying histone, CENP-A. However, the regulatory mechanisms that ensure the precise control of CENP-A deposition have proven elusive. Here, we identify polo-like kinase 1 (Plk1) as a centromere-localized regulator required to initiate CENP-A deposition in human cells. We demonstrate that faithful CENP-A deposition requires integrated signals from Plk1 and cyclin-dependent kinase (CDK), with Plk1 promoting the localization of the key CENP-A deposition factor, the Mis18 complex, and CDK inhibiting Mis18 complex assembly. By bypassing these regulated steps, we uncoupled CENP-A deposition from cell-cycle progression, resulting in mitotic defects. Thus, CENP-A deposition is controlled by a two-step regulatory paradigm comprised of Plk1 and CDK that is crucial for genomic integrity.

SUBMITTER: McKinley KL 

PROVIDER: S-EPMC4192726 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature

altmetric image

Publications

Polo-like kinase 1 licenses CENP-A deposition at centromeres.

McKinley Kara L KL   Cheeseman Iain M IM  

Cell 20140701 2


To ensure the stable transmission of the genome during vertebrate cell division, the mitotic spindle must attach to a single locus on each chromosome, termed the centromere. The fundamental requirement for faithful centromere inheritance is the controlled deposition of the centromere-specifying histone, CENP-A. However, the regulatory mechanisms that ensure the precise control of CENP-A deposition have proven elusive. Here, we identify polo-like kinase 1 (Plk1) as a centromere-localized regulato  ...[more]

Similar Datasets

| S-EPMC5602426 | biostudies-literature
| S-EPMC3265468 | biostudies-literature
| S-EPMC5097169 | biostudies-literature
| S-EPMC2824361 | biostudies-literature
| S-EPMC3207292 | biostudies-literature
| S-EPMC4945145 | biostudies-literature
| S-EPMC3103353 | biostudies-literature
| S-EPMC2193228 | biostudies-literature
| S-EPMC5710141 | biostudies-literature
| S-EPMC4878093 | biostudies-literature