Unknown

Dataset Information

0

MRNA-Seq and microRNA-Seq whole-transcriptome analyses of rhesus monkey embryonic stem cell neural differentiation revealed the potential regulators of rosette neural stem cells.


ABSTRACT: Rosette neural stem cells (R-NSCs) represent early stage of neural development and possess full neural differentiation and regionalization capacities. R-NSCs are considered as stem cells of neural lineage and have important implications in the study of neurogenesis and cell replacement therapy. However, the molecules regulating their functional properties remain largely unknown. Rhesus monkey is an ideal model to study human neural degenerative diseases and plays intermediate translational roles as therapeutic strategies evolved from rodent systems to human clinical applications. In this study, we derived R-NSCs from rhesus monkey embryonic stem cells (ESCs) and systematically investigated the unique expressions of mRNAs, microRNAs (miRNAs), and signalling pathways by genome-wide comparison of the mRNA and miRNA profilings of ESCs, R-NSCs at early (R-NSCP1) and late (R-NSCP6) passages, and neural progenitor cells. Apart from the R-NSCP1-specific protein-coding genes and miRNAs, we identified several pathways including Hedgehog and Wnt highly activated in R-NSCP1. The possible regulatory interactions among the miRNAs, protein-coding genes, and signalling pathways were proposed. Besides, many genes with alternative splicing switch were identified at R-NSCP1. These data provided valuable resource to understand the regulation of early neurogenesis and to better manipulate the R-NSCs for cell replacement therapy.

SUBMITTER: Zhao Y 

PROVIDER: S-EPMC4195499 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature

altmetric image

Publications

mRNA-Seq and microRNA-Seq whole-transcriptome analyses of rhesus monkey embryonic stem cell neural differentiation revealed the potential regulators of rosette neural stem cells.

Zhao Yuqi Y   Ji Shuang S   Wang Jinkai J   Huang Jingfei J   Zheng Ping P  

DNA research : an international journal for rapid publication of reports on genes and genomes 20140617 5


Rosette neural stem cells (R-NSCs) represent early stage of neural development and possess full neural differentiation and regionalization capacities. R-NSCs are considered as stem cells of neural lineage and have important implications in the study of neurogenesis and cell replacement therapy. However, the molecules regulating their functional properties remain largely unknown. Rhesus monkey is an ideal model to study human neural degenerative diseases and plays intermediate translational roles  ...[more]

Similar Datasets

2014-07-03 | E-GEOD-53260 | biostudies-arrayexpress
2014-07-03 | GSE53260 | GEO
| S-EPMC3180378 | biostudies-literature
| S-EPMC4936634 | biostudies-literature
| S-EPMC3117859 | biostudies-literature
2011-10-18 | GSE30510 | GEO
2011-10-18 | E-GEOD-30510 | biostudies-arrayexpress
2006-08-17 | GSE4446 | GEO
| S-EPMC5235956 | biostudies-literature
| S-EPMC5056160 | biostudies-literature