Ontology highlight
ABSTRACT:
SUBMITTER: Blaise M
PROVIDER: S-EPMC419609 | biostudies-literature | 2004
REPOSITORIES: biostudies-literature
Blaise Mickaël M Becker Hubert Dominique HD Keith Gérard G Cambillau Christian C Lapointe Jacques J Giegé Richard R Kern Daniel D
Nucleic acids research 20040518 9
Escherichia coli encodes YadB, a protein displaying 34% identity with the catalytic core of glutamyl-tRNA synthetase but lacking the anticodon-binding domain. We show that YadB is a tRNA modifying enzyme that evidently glutamylates the queuosine residue, a modified nucleoside at the wobble position of the tRNA(Asp) QUC anticodon. This conclusion is supported by a variety of biochemical data and by the inability of the enzyme to glutamylate tRNA(Asp) isolated from an E.coli tRNA-guanosine transgl ...[more]