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Similar gene expression patterns characterize aging and oxidative stress in Drosophila melanogaster.


ABSTRACT: Affymetrix GeneChips were used to measure RNA abundance for approximately 13,500 Drosophila genes in young, old, and 100% oxygen-stressed flies. Data were analyzed by using a recently developed background correction algorithm and a robust multichip model-based statistical analysis that dramatically increased the ability to identify changes in gene expression. Aging and oxidative stress responses shared the up-regulation of purine biosynthesis, heat shock protein, antioxidant, and innate immune response genes. Results were confirmed by using Northerns and transgenic reporters. Immune response gene promoters linked to GFP allowed longitudinal assay of gene expression during aging in individual flies. Immune reporter expression in young flies was partially predictive of remaining life span, suggesting their potential as biomonitors of aging.

SUBMITTER: Landis GN 

PROVIDER: S-EPMC419663 | biostudies-literature | 2004 May

REPOSITORIES: biostudies-literature

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Similar gene expression patterns characterize aging and oxidative stress in Drosophila melanogaster.

Landis Gary N GN   Abdueva Diana D   Skvortsov Dmitriy D   Yang Junsheng J   Rabin Beth E BE   Carrick James J   Tavaré Simon S   Tower John J  

Proceedings of the National Academy of Sciences of the United States of America 20040510 20


Affymetrix GeneChips were used to measure RNA abundance for approximately 13,500 Drosophila genes in young, old, and 100% oxygen-stressed flies. Data were analyzed by using a recently developed background correction algorithm and a robust multichip model-based statistical analysis that dramatically increased the ability to identify changes in gene expression. Aging and oxidative stress responses shared the up-regulation of purine biosynthesis, heat shock protein, antioxidant, and innate immune r  ...[more]

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