Ontology highlight
ABSTRACT:
SUBMITTER: Kim B
PROVIDER: S-EPMC4203659 | biostudies-literature | 2014
REPOSITORIES: biostudies-literature
Kim Borim B Lee Soojin S Jeong Daun D Yang Jeongmo J Oh Min-Kyu MK Lee Jinwon J
PloS one 20141020 10
Klebsiella pneumoniae KCTC2242 has high potential in the production of a high-value chemical, 2,3-butanediol (2,3-BDO). However, accumulation of metabolites such as lactate during cell growth prevent large-scale production of 2,3-BDO. Consequently, we engineered K. pneumoniae to redistribute its carbon flux toward 2,3-BDO production. The ldhA gene deletion and gene overexpression (budA and budB) were conducted to block a pathway that competitively consumes reduced nicotinamide adenine dinucleoti ...[more]