Ontology highlight
ABSTRACT:
SUBMITTER: Nazarewicz RR
PROVIDER: S-EPMC4210376 | biostudies-literature | 2013 Apr
REPOSITORIES: biostudies-literature
Nazarewicz Rafal R RR Bikineyeva Alfiya A Dikalov Sergey I SI
Journal of biomolecular screening 20121127 4
Superoxide plays a key role in many pathological processes; however, detection of superoxide by one of the most common methods using dihydroethidium (DHE) may be unspecific because of overlapping fluorescence of the superoxide-specific product, 2-OH-ethidium (2OH-E), and the unspecific oxidation product, ethidium. Here, we show a new optimized fluorescence spectroscopy protocol that allows rapid and specific detection of superoxide in cell-free systems and intact cells using DHE. We defined new ...[more]