Dataset Information

Molecular characterization of various trichomonad species isolated from humans and related mammals in Indonesia.

ABSTRACT: Trichomonad species inhabit a variety of vertebrate hosts; however, their potential zoonotic transmission has not been clearly addressed, especially with regard to human infection. Twenty-one strains of trichomonads isolated from humans (5 isolates), pigs (6 isolates), rodents (6 isolates), a water buffalo (1 isolate), a cow (1 isolate), a goat (1 isolate), and a dog (1 isolate) were collected in Indonesia and molecularly characterized. The DNA sequences of the partial 18S small subunit ribosomal RNA (rRNA) gene or 5.8S rRNA gene locus with its flanking regions (internal transcribed spacer region, ITS1 and ITS2) were identified in various trichomonads; Simplicimonas sp., Hexamastix mitis, and Hypotrichomonas sp. from rodents, and Tetratrichomonas sp. and Trichomonas sp. from pigs. All of these species were not detected in humans, whereas Pentatrichomonas hominis was identified in humans, pigs, the dog, the water buffalo, the cow, and the goat. Even when using the high-resolution gene locus of the ITS regions, all P. hominis strains were genetically identical; thus zoonotic transmission between humans and these closely related mammals may be occurring in the area investigated. The detection of Simplicimonas sp. in rodents (Rattus exulans) and P. hominis in water buffalo in this study revealed newly recognized host adaptations and suggested the existence of remaining unrevealed ranges of hosts in the trichomonad species.

SUBMITTER: Kamaruddin M

PROVIDER: S-EPMC4210728 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature

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Molecular characterization of various trichomonad species isolated from humans and related mammals in Indonesia.

Kamaruddin Mudyawati M Tokoro Masaharu M Rahman Md Moshiur MM Arayama Shunsuke S Hidayati Anggi P N AP Syafruddin Din D Asih Puji B S PB Yoshikawa Hisao H Kawahara Ei E

The Korean journal of parasitology 20141022 5

Trichomonad species inhabit a variety of vertebrate hosts; however, their potential zoonotic transmission has not been clearly addressed, especially with regard to human infection. Twenty-one strains of trichomonads isolated from humans (5 isolates), pigs (6 isolates), rodents (6 isolates), a water buffalo (1 isolate), a cow (1 isolate), a goat (1 isolate), and a dog (1 isolate) were collected in Indonesia and molecularly characterized. The DNA sequences of the partial 18S small subunit ribosoma ...[more]

PMID: 25352694

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Project description:ObjectiveThis study highlighted the dissemination of Bacillus species (including drug-resistant species) in public hospital environments and calls for the design of optimal strategies to curb their spread. This a critical consideration for all health care systems such as caring for the increasing number of immune-compromised patient.MethodsA total of 528 swab samples were collected from the environments of different Saudi hospitals. Swab samples were collected by swabbing approximately 5 cm2 of different surfaces at each site using pre-moisturized cotton swabs with 1 mL of neutralizing buffer. The swabs were transported in cool boxes with ice packs within 2 h of collection. Isolation and identification were performed according to conventional bacteriological, semi-automated and molecular characterization methods. Antibiogram typing was carried against different groups of antimicrobial agents.ResultsThe most prevalent of the isolated Bacillus species were Bacillus cereus (46.6%) followed by Bacillus subtilis (38.1%); the least prevalent was Bacillus pumilus (1.1%). Most Bacillus isolates (25.6%) were isolated from the Department of Internal Medicine followed by the Emergency Department (18.8%), while the operating rooms had the lowest prevalence (4.5%). Antimicrobial susceptibility testing revealed high levels of resistance in Bacillus isolates to β-lactams and tetracycline. Overall, 21.6% of isolates showed multi-drug resistance to three or more antibiotics (21.6%). Antibiogram typing of the 176 isolates revealed 45 antibiotypes; the most common was antibiotype 31, which included 32 isolates (18.2%); this particular antibiotype was resistant to both penicillin and cefoxitin.ConclusionsAnalyses identified the high dissemination of Bacillus species in several hospital environments with high resistance to β-lactams and tetracycline antibiotics. Molecular analysis also revealed the existence of genetic diversity among the Bacillus isolates investigated. Thus, monitoring the hospital environment is an important tool in the prevention of hospital-associated infection by Bacillus species.

Dataset Information

Molecular characterization of various trichomonad species isolated from humans and related mammals in Indonesia.

Publications

Molecular characterization of various trichomonad species isolated from humans and related mammals in Indonesia.

Similar Datasets

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