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Mapping biological composition through quantitative phase and absorption X-ray ptychography.


ABSTRACT: Isolating compositional information in biological X-ray imaging can be problematic as such information is conflated with thickness and density variations when viewing in projection through a sample. We demonstrate an effective method for identifying variations in material composition by simultaneously using the quantitative phase and magnitude images provided through soft X-ray ptychography. Using this approach we show significantly increased contrast and improved reliability of the identification of intracellular features from uncharacterised samples. While demonstrated for X-ray ptychography, this method is immediately applicable to electron and optical microscopy methods where the complex transmission function of the sample is recovered.

SUBMITTER: Jones MW 

PROVIDER: S-EPMC4210942 | biostudies-literature |

REPOSITORIES: biostudies-literature

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