Ontology highlight
ABSTRACT:
SUBMITTER: Hwang MS
PROVIDER: S-EPMC4211371 | biostudies-literature | 2014 Nov
REPOSITORIES: biostudies-literature
Hwang M-S MS Schwall C T CT Pazarentzos E E Datler C C Alder N N NN Grimm S S
Cell death and differentiation 20140620 11
Massive Ca(2+) influx into mitochondria is critically involved in cell death induction but it is unknown how this activates the organelle for cell destruction. Using multiple approaches including subcellular fractionation, FRET in intact cells, and in vitro reconstitutions, we show that mitochondrial Ca(2+) influx prompts complex II of the respiratory chain to disintegrate, thereby releasing an enzymatically competent sub-complex that generates excessive reactive oxygen species (ROS) for cell de ...[more]