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A novel HoxB cluster protein expressed in the hindbrain and pharyngeal arches.


ABSTRACT: We describe a novel zebrafish line that fluorescently tags a previously unknown protein, CT74a, allowing us to follow its endogenous expression in real time and at subcellular resolution in live embryos. Our results showed that CT74a-Citrine fusion protein is expressed in the developing pharyngeal arches, hindbrain, and fin buds in a pattern highly reminiscent of transcription factors belonging to anterior Hox gene families, including expression in a subset of neuronal nuclei. Consistent with this, splinkerette-PCR revealed that CT74a-Citrine's genomic integration is within the HoxB region, and 3' RACE demonstrated that its downstream coding sequence has no recognizable homology. Thus, CT74a is a previously unknown protein located within the HoxB cluster adjacent to Hoxb4a and is expressed in a Hoxb4a-like pattern.

SUBMITTER: Saxena A 

PROVIDER: S-EPMC4211940 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature

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A novel HoxB cluster protein expressed in the hindbrain and pharyngeal arches.

Saxena Ankur A   Bronner Marianne E ME  

Genesis (New York, N.Y. : 2000) 20140823 10


We describe a novel zebrafish line that fluorescently tags a previously unknown protein, CT74a, allowing us to follow its endogenous expression in real time and at subcellular resolution in live embryos. Our results showed that CT74a-Citrine fusion protein is expressed in the developing pharyngeal arches, hindbrain, and fin buds in a pattern highly reminiscent of transcription factors belonging to anterior Hox gene families, including expression in a subset of neuronal nuclei. Consistent with th  ...[more]

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