Ontology highlight
ABSTRACT:
SUBMITTER: Ishida YI
PROVIDER: S-EPMC4215121 | biostudies-literature | 2014
REPOSITORIES: biostudies-literature
Ishida Y I YI Takikawa M M Suzuki T T Nagahama M M Ogasawara Y Y
FEBS open bio 20141013
Peroxiredoxin 2 (Prx2) is the third most abundant protein in red blood cells (RBCs). In this study, we have succeeded in implementing the rapid and simultaneous detection of the hyperoxidized (Prx2-SO2/3) and reduced (Prx2-SH) forms of Prx2 in human RBCs using reverse phase high-performance liquid chromatography. The detection of a peak corresponding to Prx2-SO2/3 was clearly observed following treatment of tert-butyl hydroperoxide (t-BHP), but not H2O2, and was found to be dose-dependent. The i ...[more]